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| Labry, Claire; Delmas, Daniel; Moriceau, Brivaela; Gallinari, Morgane; Quere, Julien; Youenou, Agnes. |
| Phosphorus (P) limitation of phytoplankton growth is known to affect the accumulation and release of carbohydrates (CHO) by micro-algae. However, relatively little is known about the fate of algal exudates, notably their bacterial degradation. The CHO chemical characterization is also not exhaustive, especially in ‘functional’ pools relevant for phytoplankton physiology (particulate reserve [R] or structural [S] CHO) and for bacterial degradation (dissolved mono- [MDCHO] and polysaccharides [P-DCHO]). In this study, we investigated how P depletion and repletion affect the CHO composition in diatom Thalassiosira weissflogii cultures, and the shortterm response of free and diatom-attached bacteria in terms of abundance and potential βglucosidase activity... |
| Tipo: Text |
Palavras-chave: P limitation; Phytoplankton; Diatom; Bacteria; Mineralization; Carbohydrate; Glucosidase. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00628/74045/73532.pdf |
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| Bogas,Andréa C.; Watanabe,Maria Angelica E.; Barbosa,Aneli; Vilas-Boas,Laurival A.; Bonatto,Ana C.; Dekker,Robert; Souza,Emanuel M.; Fungaro,Maria Helena P.. |
| A beta-glucosidase-like enzyme-encoding gene (bglH) of an endophytic Bacillus pumilus strain (CL16) was cloned using a shotgun genomic library constructed in Escherichia coli. The nucleotide sequence of the entire cloned fragment (2484 bp) was determined and characterized. An incomplete open reading frame (ORF) of 534 bp (ORF1) designated bglP and a complete ORF of 1419 bp (ORF2) designated bglH, located in the fragment, are organized in an operon. The protein deduced from 1419 bp (ORF2) had 472 amino acid residues without a characteristic signal peptide sequence, suggesting that the enzyme is localized in the cytoplasm. The amino acid sequence deduced from bglH gene had high similarity with beta-glucosidases from the glycosyl hydrolase family 1.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus pumilus; BglH; Glucosidase; Glycosyl hydrolase 1; PTS. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100018 |
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